jueves, 1 de agosto de 2013

Molecular Characterization and resistance of H. influenzae isolated from Nasopharynx of Students in North Lebanon

Introduction: Haemophilus influenzae is an important cause of respiratory infections, including acute otitis media, sinusitis, and chronic bronchitis, which are preceded by asymptomatic H. influenzae colonization of the human pharynx. The aim of this study is to investigate the rate of H.influenzae nasopharyngeal colonization among students ages 2 to 3 years. Material and methods: A total of 21 isolates of clinical H. influenzae were isolated from 87 nasopharyngeal specimens of children between April and June 2011. The isolates were identified by using molecular techniques (PCR), biotypes were determined by using the following tests: ornithin decarboxylase, urease and tryptophanase, and capsular typing was performed by SAST by using polyclonal and specific b antisera (Difco-BD®-USA). The prevalence of β -lactams resistance, β-lactamase production, the level of macrolide resistance was recorded for each strain by using disc diffusion and E-test strip methods and chromogenic cephalosporin test (cefinase). β -lactams resistance genes (blaTEM and blaROB) were determined using PCR. Results: 42.8 % of the H. influenzae isolates were type b, and biotypes I, II and III were the majority, whereas biotypes IV, VI and VIII was not found. The majority of capsule type b was belonged to biotype II. Antibiotics susceptibility showed that 19% of the isolates were resistant to ampicillin and produced type TEM-1 β-lactamase. Conclusion: This study shows the carriage rate of H. influenzae in North Lebanon children. The incidence of resistance rate of 19% to ampicillin signals an important warning to the future prophylaxis use of beta-lactam in treatment of H. influenzae infections in Lebanon.

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