lunes, 5 de agosto de 2013

Fresh leafy green vegetables associated with multidrug resistant E.coli

Objective: To detect the occurrence of diarrheagenic E. coli, contamination and antibiotic resistance of E. coli isolates from fresh leafy vegetables presented in Jordanian retail markets. Materials and Methods: A total 150 fresh leafy green vegetable samples were collected from different markets in Amman and Al-Zarqa in Jordan over the five months; June through October, 2010. All samples were prepared in sterile normal saline and cultured on MacConkey agar at 37°C for 24 hours. E. coli isolates were later identified by biochemical tests and the isolates were investigated for presence of diarrheagenic E. coli types and antimicrobial resistance genes, integrons and plasmids. Results: A total of 61/150 (40.6%) of fresh leafy green vegetable samples were found contaminated with E. coli. The highest contamination with E. coli occurred in parsley and lowest in Lettuce. A range of 3-41% of E. coli isolates were resistant to 7 common antibiotics used often in Jordan. A total of 17 (27.8%) E. coli isolates were resistant to three or more antimicrobial agents and considered to be multidrug resistant. All these 17 (100%) carry at least one plasmid with a common sizes (7.3 and 54.3 Kb), 14 (82%) isolates were positive for class 1 integrons, 12 (70.6%), and 2 (11.8%) isolates were positive for Tet A and Tet B genes, respectively. A total of 15 (88.2%) isolates were positive for sul 2 and 4 (23.5%) isolates for sul1genes. Conclusion: This study demonstrates the absence of common diarrheagenic E. coli, but indicates widespread of antimicrobial resistance in E. coli contaminating fresh green produce which may increase the reservoir of antimicrobial resistance in the intestinal tract of Jordanian population.

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