sábado, 3 de agosto de 2013

Evaluation of the combination of N-acetylcysteine and or sodium salicylate with ciprofloxacin on bacterial adhesion and biofilm formation on urinary catheters

Background: Bacteria can adhere to the surface of indwelling urinary devices and forming a biofilm lead to complete blocking to the lumen of the urinary catheter and represent a site of spreading infections. Methods: This study aimed to evaluate the effect of ciprofloxacin (CIP), N-acetylcysteine (NAC) and salicylate (SAL) alone and in combinations on both biofilm production and pre-formed mature biofilms on microtiter plates and urinary tract catheter segments. Results: Ciprofloxacin at MIC, 1/2MIC, 1/4MIC and 1/8MIC inhibited biofilm production on microtiter plates by 74.36%, 69.55%, 52.41% and 42.37%, respectively and disrupted the pre-formed biofilms by 53.77%, 47.72%, 42.13% and 34.78%, respectively. Ciprofloxacin also inhibited the biofilm production on catheter segments by 67.35% and 55.22% in presence of MIC and 1/4MIC, respectively and disrupted the pre-formed biofilms by 65.83% in presence of MIC and 51.55% in presence of 1/4MIC. NAC and SAL each alone inhibited biofilm production by up to 71% and 68% on microtiter plates and by up to 62% and 68% on catheter segments, respectively and disrupted pre-formed biofilms by up to 67% and 62% from microtiter plates and by up to 61% and 62% from catheter segments, respectively. Ciprofloxacin / N-acetylcysteine showed higher inhibitory effect on biofilm production and higher disruptive effect on the pre-formed biofilms than ciprofloxacin / Salicylate. When salicylate was added to the ciprofloxacin / N-acetylcysteine combination it enhanced the biofilm inhibition to become up to 99% and the disruptive effect became up to 98%. Conclusion: our results revealed the great effect of the tested combinations on inhibition of bacterial adherence and in removal of pre-formed biofilms.

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