domingo, 18 de agosto de 2013

Detection of metallo-β -lactamase in Pseudomonas aeruginosa from Tripoli, Libya

Metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa isolates have been reported to be an important cause of nosocomial infections. As very little information of the antibiotic resistance in Libya is known, this work was undertaken. The present study was conducted to study the incidence of MBLs in P. aeruginosa isolated from patients admitted to Tripoli Medical Center (TMC) and to Burn and plastic Surgery Hospital (BPSH). Three hundred and twelve isolates of P. aeruginosa were cultured from different samples from patients. Isolates were identified using Conventual’s method and API20 NE. The isolates were subjected to susceptibility testing using CLSI guidelines. They were further screened for the production of MBLs by disc potentiating testing using EDTA-impregnated imipenem discs. Of the total 312 isolates of P. aeruginosa, antimicrobial susceptibly tests to fifteen anti-pseudomonal antibiotic showed that the percent of resistance was as follows; Amikacin (23.1%), Aztreonam (22.8%), Carbenicillin (51.9%), Cefepime (28.2%), Cefotaxime (51%), Ceftazidime (29.5%), Ciprofloxacin (26.9%), Gentamicin (35.3%), Imipenem (13.8%), Meropenem (15.7%), Piperacillin (39.1%), Piperacillin/ tazobactam (27.6%), Polymyxin B (13.8%), Ticarcillin (53.8%), Tobromycin (31.1). Thirty four of imipenem resistant isolates were found to be MBL positive (10.9%). The study showed that MBL screening tests are simple and easy to perform as routine diagnostic tests for the detection of MBL production. EDTA disk screening test is simple to perform and to interpret and can easily be introduced into the workflow of a clinical laboratory. We recommend that all IPM non susceptible P. aeruginosa isolates be routinely screened for MBL production using the EDTA disk screen test and that PCR confirmation be performed at a regional laboratory

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