lunes, 5 de agosto de 2013

Comparative Study of Wound Healing After Treatment With Crude Date Extract and Silver Sulphadiazine

Objective: Various naturally occurring substances have been used in wound treatment throughout history. The most common topical agent used in the treatment of wounds includes silver sulphadiazine. Crude date extract (CDE) in comparison with silver sulphadiazine was tested to evaluate their effects on wound healing and their antimicrobial activities against the growth of Staphylococcus aureus. Material and Methods: The effect of different concentrations of crude khudari date extract on the growth of S. aureus was studied. Active compound was partially purified using thin layer chromatography technique with different solvents. Evaluation of wound healing activity with CDE was done by creating full-thickness incision wounds in rabbit’s skin and using in comparison silver sulphadiazine. Results: Crude date extract 5% (w/v) and silver sulphadiazine 20% (w/v) inhibited the growth of S. aureus by 67% and 87%, respectively. However, 10% and 20% (w/v) date extract showed 100% inhibition. Date extract was fractionated using different solvents with different polarities; all fractions were tested for their antimicrobial activities. Ethyl acetate fraction was found to have inhibitory activity against the growth of S. aureus. Preparative TLC was done for ethyl acetate fraction which was further identified to be terpenoidal compound. The histological changes in healing wounds have also been investigated by light microscopy after treatment with 20% and 40% crude date extract, silver sulphadiazine and amoxicillin. Conclusions: The results of this study showed that 20% CDE treatment has promoted the process of wound healing and stimulates fibroblast, collagen and epithelialization significantly. This effect was comparable with the effect of silver sulphadiazine treatment

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