lunes, 5 de agosto de 2013

A novel binary biofilm model for the study of the development of antimicrobial tolerance in Pseudomonas aeruginosa PAO1 and Escherichia coli

The primary aim of this study was to investigate the effect of species interactions on biofilm formation and the investigation of the susceptibility of component species towards the antimicrobial agent Benzisothiazolone (BIT) in binary biofilms (Ps. aeruginosa PAO1and E. coli ATCC 10000). The Sorbarod biofilm model [1] was used to establish mono-species and binary species biofilms under conditions of growth rate control by the utilisation of a Modified Chemically Defined Medium (MCDM). This MCDM was designed to allow the growth of both bacterial species (Ps. aeruginosa PAO1and E. coli ATCC 10000) in the same biofilm, whilst preventing either species from having a growth rate advantage over the other. Our results suggest that it is possible to use this model to investigate the consequences of environmental exposure of bacteria to sub-MICs of biocides and develop a comprehensive insight of their subsequent tolerance and resistance characteristics. These results indicate that it is possible to establish a binary biofilm in a modified chemically defined media, subjected to growth rate control and to induce tolerance in dual species (binary) biofilms in response to BIT. The mechanism of tolerance in binary biofilms towards this biocide was a gradual adaptive process, dependent upon the presence of the biocide itself. This study elucidates a novel technique for the establishment, control and operation of binary biofilms. It has yielded information regarding the use of passage approaches to develop antimicrobials tolerance in both mono- and binary species biofilms of medically important bacteria.

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